c reconstitution buffer (Nitta Gelatin Inc)

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Nitta Gelatin Inc c reconstitution buffer
C Reconstitution Buffer, supplied by Nitta Gelatin Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reconstitution+buffer/buffer+c+reconstitution/pmc13138982-76-3-6
Average 86 stars, based on 1 article reviews

c reconstitution buffer - by Bioz Stars, 2026-11

86/100 stars

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Article Title: FGF‐stimulated tendon cells embrace a chondrogenic fate with BMP7 in newt tissue culture
Article Snippet: To adhere the tissue to the surface of the wells, they were embedded in collagen gel made of Type IA collagen (Nitta Gelatin Inc.), 10× αMEM (Wako‐Fuji film), and reconstitution buffer (Nitta Gelatin Inc.) and then kept stationary in an incubator for 30 min at 25°C with 5.0% CO 2 .

Article Title: FGF-stimulated tendon cells embrace a chondrogenic fate with BMP7 in newt tissue culture.
Article Snippet: To adhere the tissue to the surface of the wells, they were embedded in collagen gel made of Type IA collagen (Nitta Gelatin Inc.), 10 αMEM (Wako-Fuji film), and reconstitution buffer (Nitta Gelatin Inc.) and then kept stationary in an incubator for 30 min at 25 C with 5.0% CO2.

Construct:

Article Title: β-endorphin suppresses ultraviolet B irradiation-induced epidermal barrier damage by regulating inflammation-dependent mTORC1 signaling
Article Snippet: .. Normal human dermal fibroblasts (5 × 10 4 cells per well; Lonza) were used to construct the dermal layer by mixing cell matrix type I (Nitta Gelatin Inc., Osaka, Japan) in reconstitution buffer (Nitta Gelatin Inc.). .. The mixture of fibroblasts was transferred to each insert of a six-well plate (Snapwell; Corning, NY, USA) and then incubated at 37 °C for 2 h for polymerization.

Article Title: β-endorphin suppresses ultraviolet B irradiation-induced epidermal barrier damage by regulating inflammation-dependent mTORC1 signaling.
Article Snippet: .. Normal human dermal fibroblasts (5 × 104 cells per well; Lonza) were used to construct the dermal layer by mixing cell matrix type I (Nitta Gelatin Inc., Osaka, Japan) in reconstitution buffer (Nitta Gelatin Inc.). .. The mixture of fibroblasts was transferred to each insert of a six-well plate (Snapwell; Corning, NY, USA) and then incubated at 37 °C for 2 h for polymerization.

Suspension:

Article Title: Inhibitor for retinochoroidal disorders
Article Snippet: After washing twice in a serum free medium (MEM: product number 11095; Gibco), a serum-free medium was added to make a cell suspension. .. Type I collagen (3 mg/ml: product number 637-00653; Nitta Gelatin Inc.), 10×MEM, reconstitution buffer (product number 635-00791; Nitta Gelatin Inc.), cell suspension (1.1×107 cells/ml in MEM), and water were mixed on ice at the volume ratio of 7:1:1:0.2:1.8. ..

Concentration Assay:

Article Title: Therapeutic agent for keratoconjunctive disorders
Article Snippet: After washing in a serum free medium (product number 11095; Gibco), the number of cells were counted. .. The obtained primary keratocytes were mixed with the type I collagen solution Cellmatrix Type I-A (product number 637-00653; Nitta Gelatin Inc.) and Reconstitution Buffer (product number 635-00791; Nitta Gelatin Inc.) and dispensed into a 24-well plate so that the final concentration would be 1×105 cells/well to make a collagen gel. .. After making the gel, culture was started (under conditions of 37′C and 5% CO2) by adding to a MEM medium a RARγ agonist A (R667)-containing dimethyl sulfoxide solution (R667 concentration: 0.1 nM, 1 nM, 10 nM, 100 nM, or 1000 nM) or a RARγ agonist-free dimethyl sulfoxide solution as a control and 10 ng (final concentration 10 ng/ml) of IL-1β (product number 201-LB-005; R&D Systems) and 60 μg (final concentration 60 μg/ml) of Plasminogen (product number P9156; Sigma) as a stimulant, and overlaying the medium on the previously prepared collagen gel.

Cell Culture:

Article Title: Activation of the Sphingosine 1 Phosphate–Rho Pathway in Pterygium and in Ultraviolet-Irradiated Normal Conjunctiva
Article Snippet: .. Resected pterygium or normal conjunctiva tissue were embedded in Cellmatrix Type I-A (Nitta Gelatin Inc., Osaka, Japan) mixed with Ham’s F-12 medium and reconstitution buffer (both Nitta Gelatin Inc.), at an 8:1:1 ratio, and placed in a 35-mm cell culture dish. .. Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS) and antibiotic antimycotic solution (100×) (Sigma-Aldrich 136 Co., LLC, St. Louis, MO, USA) were added to cover the tissue.

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